Version 63 (modified by 14 years ago) (diff) | ,
---|
Table of Contents
Port applications to Dutch Life Science Grid
People
- AMC: Antoine van Kampen, Barbera van Schaik, Silvia D Olabarriaga, Mark Santcroos
- Sara/BiGGrid: Tom Visser
- UMCG: Morris Swertz, Freerk van Dijk
Description Software is going to be implemented as workflow components. The workflows will run on the Dutch life science grid.
- Information about the infrastructure: http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/
- Getting started: http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/GettingStarted
- Angela CM Luyf, Barbera DC van Schaik, Michel de Vries, Frank Baas, Antoine HC van Kampen, Silvia D Olabarriaga (2010) Initial steps towards a production platform for DNA sequence analysis on the grid. BMC Bioinformatics 11: 1. Dec pubmed
Implemented workflow components at AMC
This list of workflow components are already available. We can expand it with Pindel and (parts of) the GATK pipeline.
- Splitting of fastq files
- Building a BWA index on the genome sequence (base space and color space)
- BWA for shotgun reads (base space and color space) It is possible to do parameter sweeps. Output is in bam format
- Merge bam results
- Samtools pileup
- Varscan (pileup to snp, indel and cns)
- Bam2coverage creates a UCSC wiggle file to display the genome coverage (per 50kbp)
- Coverage-per-base determines the coverage for every base in the genome and it summarizes the results (coverage versus frequency)
- Annovar (works for hg18, working on other assemblies). This is a pipeline to annotate variants (gene, dbsnp, hapmap, 1000g, conservation, etc)
- FastqC
Implemented components of the Groningen pipeline A more detailed description will follow later
- BwaIllumina (done) - pe00-bwa-align-pair1.ftl, pe01-bwa-align-pair2.ftl, pe02-bwa-sampe.ftl, pe03-sam-to-bam.ftl, pe04-sam-sort.ftl
- MarkDuplicates (done) - pe05-mark-duplicates.ftl
- PicardQC (partly done) - pe04b-picardQC.ftl. Didn't get the R environment up and running yet, so the .pdf .hist and .bamindexstats can not be produced yet. Will continue with the other components and fix this later. Attachment contains info about the required R packages.
- GatkGenerateIntervalFile (done) - see e-mail Freerk on Dec 13, 2010
- ReAlign (in progress) - pe06-realign.ftl. Fails, need to discuss this with Freerk
- FixMates (done) - pe07-fixmates.ftl
- GatkCovariates (done) - pe08-covariates-before.ftl, pe11-covariates-after.ftl
- GatkRecalibrate (done) - pe09-recalibrate.ftl
- SortSam (done) - pe10-sam-sort.ftl
- AnalyzeCovariates (done) - pe12-analyze-covariates.ftl
- Update 31-1-2011: integrating all the components in two workflows; one for alignment, one for the subsequent steps
To be implemented
- The components of the Groningen pipeline that not implemented as a workflow component yet
- Pindel
Data access rights
To ensure that the most limited group of people has access to the data we have created a subgroup "gvnl" within the "vlemed" Virtual Organisation (VO). For people to become part of this group, it is required that they have a Grid certificate and that they are part of the "vlemed" VO. On the following page there is information on how to get a certificate, how to get into the "vlemed" VO: http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/EBioInfra#Access
For more information about data access see http://www.bioinformaticslaboratory.nl/twiki/bin/view/EBioScience/DataManagement
Things to address
- Available disk space on the grid storage elements / worker nodes
Data location on grid
Data
The data is located on the storage element at Sara and only readable and writable for the vlemed/gvnl group. This screencast demonstrates how to access the data from the Vbrowser: http://www.youtube.com/watch?v=FicwWGAbubQ
Storage location (resource): srm.grid.sara.nl
Path: /pnfs/grid.sara.nl/data/vlemed/gvnl
Workflows and databases
These directories are open to all members of the vlemed VO
- Workflows: lfn://lfc.grid.sara.nl:5010/grid/vlemed/AMC-e-BioScience/Sequence_WF
- Databases: lfn://lfc.grid.sara.nl:5010/grid/vlemed/AMC-e-BioScience/Sequence_DB
The directories that contain the workflows have the following structure:
Directory | Description |
bin | dependent binaries like bwa, samtools |
GasW | component description, describes which executable has to run on grid and it specifies the in and output files/parameters |
Java | dependent jar files, e.g. the GATK jar |
parameterFiles | text files that contain exactly one line with the parameters that you would like to provide to bwa or another component |
Scufl | old workflow description files, can be ignored |
shFiles | these files are executed on the grid and described by the GASW descriptor, the gvnl shFiles are based on the Groningen templates |
Workflows | Workflow descriptions, clicking on them will start the Moteur plugin. Input files/parameters can be specified in the fields. If you click on "Run" the jobs are submitted to the grid |
The shell files can (in most cases) run on any linux cluster. In that case you need to place the shell file and the dependent executable(s) in one directory. At the start of each shell file is an example on how to run them.
Workflow execution
On mini pilot. Split lines-per-file: 8,000,000. Start: 18-12-2010 16:40
Lane | split BWA merge | Comments | Elapsed time (s) |
A4a_L4_HUModqRBUDIBAPE | done | 22740 | |
A4a_L6_HUModqRADDIAAPE | done | 37750 | |
A4a_L7_HUModqRBVDIBAPE | failed | ||
A4b_L3_HUModqRAFDIBAPE | done | 32256 | |
A4b_L6_HUModqRBTDIBAPE | failed | ||
R2A _L1_HUModqRADDIBAPE | failed | ||
R2A _L1_HUModqRAFDIBAPE | done | 35819 | |
R2A _L5_HUModqRAEDIAAPE | failed | ||
R2B _L3_HUModqRBTDIAAPE | done | 23754 | |
R2B _L4_HUModqRBUDIAAPE | failed | pair 1 not in correct gzip format? | |
R2B _L6_HUModqRBTDIBAPE | failed | ||
R2C _L2_HUModqRBUDIBAPE | done | 40763 | |
R2C _L2_HUModqRBVDIBAPE | failed | ||
R2C _L7_HUModqRBVDIAAPE | done | 39374 | |
Unknown_L6_HUModqRBUDIAAPE | done | 30307 |
Note: The Merge component is red (marked as failed) in the finished workflows. The bam files where produced successfully.
BWA alignment on mini pilot without splitting the data
- 19-12-2010 14:00 done - elapsed time 325886 s
PicardQC on finished bam files
- 20-12-2010 11:30 done - elapsed time (10 bam files) 8730 s
- Info runtime and used disk space (ods)
Coverage-per-base on finished bam files
- 20-12-2010 12:40 done - elapsed time 89634 s
Mark-duplicates on finished bam files
- 20-12-2010 12:47 done - elapsed time 10614 s
Re-align on finished bam files
- 20-12-2010 17:48 done - elapsed time 9165 s
Gvnl pipeline on remaining samples (split, bwa, merge, coverage-per-base, picard-qc, mark-duplicates, re-align) Start: 20-12-2010 18:50
Lane | Progress | Elapsed time |
A4a_L7_HUModqRBVDIBAPE | done | 208542 |
A4b_L6_HUModqRBTDIBAPE | done | 74476 |
R2A _L1_HUModqRADDIBAPE | done | 72636 |
R2A _L5_HUModqRAEDIAAPE | done | 55242 |
R2B _L4_HUModqRBUDIAAPE | failed | |
R2B _L6_HUModqRBTDIBAPE | done | 227859 |
R2C _L2_HUModqRBVDIBAPE | done | 44730 |
- Note1: Workflow stops after merge-bam. Working on it.
- Note2: The Dutch grid seems very busy at the moment. That could explain the longer execution times.
Gvnl pipeline on theoretical reads
- 22-12-2010 19:14 done - elapsed time 38587 s
- Workflow does continue after the merge-bam now
- To do: implement recalibration step, and a check on all bam files of the split input before the merge step
Alternatives
Clusters
- Groningen
- Description here about code template and automatic PBS script generation. Job submission/monitoring
- Leiden
- Huygens
- Lisa
- Philips
- DAS
Grid
Attachments (3)
-
r-environment.txt (134 bytes) - added by 14 years ago.
info about R packages
- log-picardqc-20101220.ods (17.1 KB) - added by 14 years ago.
- log-fastqc20110423.xls (118.0 KB) - added by 13 years ago.
Download all attachments as: .zip