AnnotateVarianListFileViaSeattleSeqAnnotation

Annotate Files with Variants through Seattle Seq Annotation: ​http://gvs.gs.washington.edu/SeattleSeqAnnotation/ . The java code that wraps the forms is provided from SeattleSeq? Annotation: ​http://gvs.gs.washington.edu/SeattleSeqAnnotation/SubmitSeattleSeqAnnotationAutoJob.java . This method wraps the wrapper(..) and provides a python implementation. In order to run there should be a directory under the current path, named "jars" with the following jar files:

• httpunit.jar
• js-1.6R5.jar
• junit-3.8.1.jar
• nekohtml-0.9.5.jar
• xercesImpl-2.6.1.jar

Parameters

For a complete list of parameters please check the ​Annotation website and the example below

Example

Code highlighting:

AnnotateVarianListFileViaSeattleSeqAnnotation(
        inputFile=/Users/alexandroskanterakis/Data/SNP/chr1.snp.Q20.gff,
        outputFile=/Users/alexandroskanterakis/Tools/annotation/seattleseqannotation/output.txt,
        eMail=alexandros.kanterakis@gmail.com,
        fileFormat=GFF,
        geneData=CCDS2008,
        allelesMaq=true,
        allelesDBSNP=true,
        scorePhastCons=true,
        scorePhastCons=true,
        consScoreGERP=true,
        chimpAllele=true,
        CNV=true,
        geneList=true,
        HapMapFreqType=HapMapFreqMinor,
        geneList=true,
        hasGenotypes=true,
        dbSNPValidation=true,
        repeats=true,
        geneList=true,
        proteinSequence=true,
        polyPhen=true,
        clinicalAssociation=true
        )

Source Code

​http://www.bbmriwiki.nl/svn/SequenceAnnotation/AnnotateVarianListFileViaSeattleSeqAnnotation/AnnotateVarianListFileViaSeattleSeqAnnotation.py

Pipeline Elements

This section will describe all parts of our annotation pipeline, the scripts have, and the features it will return.

script	feature	description	source
1KGannotation.py	alleleFreq	allele freq in 1KG	1KG
TODO

End of Pipeline

Related work

• ​http://www.svaproject.org/
• ​http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1001074